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| Pérez Bueno,Tania; Sánchez Miranda,Lilian; Rosado Ruiz-Apodaca,Ileana; Díaz Bravo,Tito; Rodríguez Edreira,Mercedes; Martínez Marrero,Siomara. |
| The composition of a culture medium produced in Cuba to grow Mycoplasma gallisepticum was evaluated using a response surface methodology. Initially, the influence of Mycoplasma base, horse serum and yeast extract on biomass production was assessed in a 2³ factorial experimental design. Then, a central composite design was applied. Mycoplasma base and horse serum factors were selected for this study. In 7 L and 35 L bioreactors, the variations carried out in the culture medium composition were estimated. The experimental results were fitted to a second order polynomial model equation. Finally, the optimal growth conditions established were at concentrations of 1,88% Mycoplasma base and 18,16% horse serum (v/v) with 0,6% of yeast extract. For the conditions... |
| Tipo: Journal article |
Palavras-chave: Culture medium optimization; Mycoplasma gallisepticum; Statistical experimental design. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200009 |
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